REPAROX

Winning project of the 2025 call for projects.

OBJECTIVE

The REPAROX project aims to understand and harness repair mechanisms of [Fe-S] enzymes, key biocatalysts in biotechnology whose production and activity are severely limited under aerobic conditions.

APPROACH

REPAROX builds on the recent discovery of a novel [Fe-S] repair mechanism, involving:

• An intrinsic component linked to the structure of the NADA enzyme
• An extrinsic component associated with the mitochondrial environment

The project combines:

• Genetics, molecular biology, and structural biology
• Computational protein design
• Extension to other [Fe-S] enzymes and to engineered metabolic pathways in yeast

IMPACT

REPAROX aims to overcome a major bottleneck in the aerobic bioproduction of [Fe-S] enzymes, enabling the development of more robust and tailor-made biocatalysts for industrial applications.

Project duration:
2026 - 2030

Scientific supervisor:
Jean Marie FRANCOIS (INSA Toulouse)

Partner institutions